FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100809
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG1140.11308514120763027No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTTA100.0070814737145.06
ACTCTAC259.362104E-487.06
GTCTATA259.362104E-487.01
TTAGAGA300.001930678772.54
CCGATCT6850.067.737224
CTTCCGA7100.064.3309861
TTCCGAT7100.064.3309862
CGATCTT1150.063.043485
CATACAG350.003557209462.1428575
CGATCTG3650.061.5753445
TCCGATC7600.061.0526353
CTGAGCA502.0015452E-458.09
ACACATA651.0865666E-555.76923145
CCAATGT400.006035175654.3753
TATGCGT400.006035175654.3756
TATAACT400.006035175654.3752
TCTATAA553.2051123E-452.7272762
CGATCTA1405.456968E-1251.7857135
TAGACAG450.00961420448.3333325
ATGTGGT450.00961420448.3333327
Produced by FastQC (version 0.11.2)