FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106376
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2450.2303151086711288No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2130.20023313529367529No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1810.1701511619162217No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC1770.16639091524404No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1720.1616906069038129No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC1710.16075054523576748No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1590.1494698052192224No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT1400.13160863352635932No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1370.12878844852222307No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT1350.1269083251861322No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG1220.1146875235015417No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1210.11374746183349628No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC1130.10622696848913288No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC1100.10340678348499661No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1100.10340678348499661No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC1080.10152666014890577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG1050.082.857155
CTTCCGA2350.080.212771
CCGATCT2450.076.9387744
TTCCGAT2550.073.921572
TGTACAG451.1889772E-464.444445
CGATCTC1152.4483597E-950.4347845
GCCTTAA450.00961564848.3333321
GTACAGC450.00961564848.3333326
GTGCTCT1203.5688572E-948.3333321
TCCGATC4150.045.4216843
GGGCGAC803.712046E-545.31257
TCTTCCG1201.9512845E-742.2916685
CCTATAA1201.9512845E-742.2916681
GGATATC907.437361E-540.277781
GGCGACC951.0227072E-438.157898
CGATCTT951.0227072E-438.157895
GACAGCG1553.4206096E-837.4193537
CTATAAG1406.535938E-736.252
GATCGTC2451.8189894E-1235.5102047
CTGATCG2501.8189894E-1234.85