FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120451
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1440.11955068866177948No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1400.11622983619895227No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1240.10294642634764344No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG100.007083905144.995011
TTAGGTT300.001931860172.497515
GTATAAG300.001931860172.497511
CTTCCGA2600.066.920781
GGGGATC350.003559378262.1407248
TTCCGAT2800.062.1407242
TGCTATA502.0034617E-457.998012
TATACTT502.0034617E-457.998015
CGATCTG1550.056.1271065
CCGATCT3000.055.5814254
GACTGTA400.006038845554.373137
CGATCTA701.6873604E-551.7839365
ACTACCG450.009620029548.3316735
ATCTGGG450.009620029548.3316737
GATCTGC1401.3971658E-841.427156
TACACCA700.001053118441.427155
TATATTC907.4447635E-540.2763943
TCTACGC750.001478455738.665348
TCCGATC4550.038.2404443
GATCTGG1001.384477E-436.2487566
Produced by FastQC (version 0.11.2)