FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420020
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10600.25236893481262795No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9550.2273701252321318No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC8090.1926098757202038No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7880.18761011380410456No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7350.17499166706347316No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7330.1745154992619399No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6370.15165944478834342No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5290.12594638350554735No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG5150.12261320889481453No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5090.12118470549021476No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5020.11951811818484834No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5000.11904195038331508No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT4960.11808961478024856No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT4910.11689919527641542No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4850.11547069187181563No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4690.11166134945954954No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT4520.10761392314651683No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG4380.104280748535784No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC4230.10070949002428456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT8550.074.636982
CTTCCGA8800.073.358131
CCGATCT8800.072.507974
CGATCTG5150.070.396095
GTCGCGC1950.052.050818
GGTCGCG2300.044.130047
TCCGATC14550.043.8588453
TCGCGCG2100.041.42829
TATAGGT2650.038.310663
AGGTCGC2750.036.913156
GTCTAGA1406.573355E-736.266941
ACTATAC1001.3882035E-436.2583053
CTGCGCG1803.1905074E-936.2496729
ATCTGGG1406.5979293E-736.2496727
CGATCTA2252.0008883E-1135.4483455
TTAGACA1301.6512622E-533.4652184
ATCTAGC1301.6526086E-533.4612357
GGGCGAC3500.033.1425557
TCTGCGC1102.4338857E-432.9542478
GGGCGCT900.003626014332.221939
Produced by FastQC (version 0.11.2)