FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2f10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2f10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455901
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7950.17437996407114703No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7920.17372192647087853No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6990.1533227608625557No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6970.15288406912904337No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6240.1368718208558437No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5980.13116882832018356No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5450.11954349738210708No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG5240.11493723418022773No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4940.10835685817754293No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT4650.1019958280416143No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4640.10177648217485813No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4590.10067975284107733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT9100.062.945754
TTCCGAT9450.061.388432
CTTCCGA9950.058.316381
GTATAAA1351.891749E-1048.3539961
CTGGGCG803.7316866E-545.3119859
CGATCTG6250.045.244455
GGGTAGG657.3169434E-444.634461
TCGCGCG2800.044.0173579
GTCGCGC2900.039.9995468
CCTACAC3450.039.9314653
GGTCGCG3000.038.6662257
TCCGATC15500.037.8908233
TTATATA1354.955382E-737.6004142
CGATCTA1951.6916601E-1037.1831445
GTACAGG1604.5631168E-836.26551
GTCTAGG800.00203118836.26551
TACAGGG1051.849875E-434.5309942
GACGTAT1051.8510681E-434.527206145
TCCCCCC3150.034.523428
CCCCCCG3000.033.8329479
Produced by FastQC (version 0.11.2)