FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304594
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4530.14872256183641175No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC4300.14117152668798466No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4190.1375601620517804No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3800.12475623288705621No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT3360.11031077434223918No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3350.10998246846622062No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3160.10374465682186781No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT3060.10046159806168212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG3050.054.6694075
CCGATCT4950.054.1892174
TTCCGAT5000.053.6473272
AGGGGGC450.00963464648.3309255
TATAATA450.00963464648.3309252
CCTAATA604.9408444E-448.330922
CTTCCGA5600.047.9072651
GTATTAC803.7259808E-545.3176841
CTTAATA803.729597E-545.310243
TAATACA657.330317E-444.6131634
TCCCCCC1652.7284841E-1143.9372028
CCAGGGG700.001055741741.4265063
CCCCCCG1859.640644E-1139.1872379
GTGCTCT3150.039.131461
GTACGGG750.001480931938.671091
TCTTCCG3200.038.5137065
CGTACAC951.0275091E-438.155993
TATATAA1001.3895854E-436.2481962
TACGGGG800.002034893336.2481962
GTACCGT1051.8512402E-434.522096