FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331632
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5890.17760650359434554No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5770.17398803493028417No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5280.15921262121870025No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5210.15710184783133113No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5130.15468953538862354No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC4950.1492618323925315No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT4260.1284556375741786No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4150.12513870796545568No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC4050.12212331741207122No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT4030.12152023930139431No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3790.11428330197327156No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3690.1112679114198871No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG3660.11036329425387177No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC3600.10855405992184108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT6250.071.918514
CTTCCGA6200.071.339921
TTCCGAT6300.070.196952
CTAGACG701.693715E-551.7846374
CTAGGGG701.693715E-551.7846373
TAGACTA604.940776E-448.332335
ACTAGGG450.0096342948.332332
TAGGGGA604.940776E-448.332334
ACTCCCC3050.045.1629946
TCCGATC9800.045.1266143
CGATCTG4250.044.352025
TCCCCCC3150.043.7292488
CCCCCCG3300.041.7415549
ATACTCC3500.041.427714
CCTAGAC700.001055726841.4277083
CCAGGGC1053.8169146E-641.4277083
CTAGGGA907.472525E-540.2769433
GCTAGAC750.001482106338.6658633
TATAGAA750.001482106338.6658632
CTCCCCC3700.037.2289547
Produced by FastQC (version 0.11.2)