FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155669
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG22050.1907985764089891No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG17510.15151397156106117No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13830.11967094384291695No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC12860.11127753708025394No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC12530.10842204818161602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT86950.055.0442772
CCGATCT86250.054.900094
CGATCTG40550.054.8992655
CTTCCGA89600.053.5874331
TCCGATC95450.050.2184563
CGATCTA16750.041.1270755
GATCTGG17450.040.3083766
GATCTGA13400.039.5036356
CGATCTC33200.038.222495
TATAGTG1406.601149E-736.2567635
TTATACT2451.8189894E-1235.5168274
ATCTAGC3950.034.8723757
ATCTGAC9750.034.204117
GATCTAG7350.033.543676
ATCTAAC2705.456968E-1232.221267
GTGTTAA14950.031.5344431
TCTAGGC1851.644421E-731.3504168
TTATACC4750.030.528046145
GATCTTA4100.030.0665826
TGCTCTT53150.030.0163042
Produced by FastQC (version 0.11.2)