FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138457
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2510.18128371985526193No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2110.15239388402175402No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2110.15239388402175402No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1960.1415601955841886No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1850.13361549072997392No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1840.13289324483413623No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG1590.11483709743819381No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1580.11411485154235611No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1500.10833688437565454No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGG300.001932228372.499823
CGATCTA451.1902572E-464.444285
CGTCTTA350.003560053262.142704145
CCGATCT2450.059.1835254
CTTCCGA2500.055.1197741
GTATAAG450.00960810848.3506741
CGATCTG1509.094947E-1248.3332145
TCTGCGC450.00962183548.333218
TATATAG450.00962183548.333212
CTGCGCA450.00962183548.333219
GGTATCC450.00962183548.333213
TATAGAG450.00962183548.333212
TTCCGAT2900.047.4998782
CTGCGCG750.001478989538.6665739
CCTGGGG750.001478989538.6665733
TACCGTC1951.6734703E-1037.1793947
TAGTTCA800.002030596636.249919
TATACTC800.002030596636.249913
ATACCGT1854.127287E-935.270186
CTATAAG1251.25726965E-534.7999152