FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298650
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5190.1737820190858865No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4830.1617277749874435No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4270.1429767286120877No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4150.13895864724594006No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3930.131592164741336No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3760.12589988280596015No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC3100.103800435292148No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2990.10011719403984598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA5200.061.364361
CCGATCT5700.055.9721454
TTCCGAT5850.054.5369642
CGATCTG4650.049.898935
CCTACGT450.00962764748.3395843
TATACCG657.3236204E-444.6211555
CTGCGCA700.001055648141.426999
TTGCGAC1105.2472315E-639.550574
GGGCGAC1651.3587851E-939.543957
GTGTATA750.001479559138.6781431
GTATAAT750.001479559138.6781431
TCTTCCG3800.038.162835
TCGCGCG1354.95731E-737.591169
GTGCTCT4300.035.417491
TATACTG850.002737184534.1220635
ACTGGGC850.00273943334.116358
TATAGGT2154.838512E-1033.7252923
ATAGCGC1301.6494525E-533.4658666
CCCCCCG1551.4608886E-632.7406859
TCCCCCC1551.4608886E-632.7406858
Produced by FastQC (version 0.11.2)