FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708520
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC23860.3367583131033704No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG21880.3088127364082877No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA16430.23189183085869136No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC15460.21820132106362558No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT15450.21806018178738779No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC14310.20197030429627955No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG14280.20154688646756622No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA12210.17233105628634338No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11340.1600519392536555No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT11160.15751143228137526No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA11120.1569468751764241No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC10920.15412408965166827No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC10700.15101902557443686No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10680.15073674702196127No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC10500.14819624004968102No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT10410.1469259865635409No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG10150.14325636538135833No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG10080.1422683904476938No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10040.1417038333427426No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT9960.14057471913284028No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG9700.13690509795065772No Hit
GATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA9190.12970699486253034No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC9160.129283577033817No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC8740.12335572743182974No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA8560.1208152204595495No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC8400.11855699203974482No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT8220.11601648506746458No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC8150.11502851013380003No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT7990.11277028171399538No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA7870.11107661039914188No Hit
AACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCT7650.10797154632191046No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG7620.10754812849319709No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC7510.10599559645458138No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG7250.10232597527239881No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG7090.10006774685259413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA52600.069.361721
TTCCGAT52600.069.209162
CCGATCT53700.067.5118564
CGATCTG24900.062.6070755
TCCGATC63650.057.5358543
CGATCTA10950.056.946715
CGATCTC19400.050.830165
GTACTAT1307.3159754E-944.635891
TATACTA657.3261827E-444.626442
GATCTAG4550.044.620146
GATCTGA7600.042.932216
ATCTAGC2550.042.6455847
ATCTGTG2400.042.2902037
GATCTAA3650.041.7167786
CGATCTT11400.041.3421255
GATCTCA5850.040.9017946
ATCTGAC3550.040.843667
GATCTGG10000.039.879256
ATCTGCG5650.039.7773867
ATATGGT750.001482317538.6707884
Produced by FastQC (version 0.11.2)