FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520029
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9280.17845158635383795No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7880.15153001082631928No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7390.14210745939168778No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7020.1349924715737007No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6910.13287720492510996No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6550.12595451407517658No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5910.11364750811973948No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5500.10576333242953759No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5300.1019173930684635No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT5250.10095590822819496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT9400.067.1053852
CTTCCGA9450.066.769591
CCGATCT9800.065.106234
CGATCTG5550.056.1747865
CGATCTA3150.048.336445
GTCGCGC2600.039.037228
TCGCGCG2700.037.5913969
GTATATA2550.036.9738271
TCCGATC17500.036.4594883
TATAGGT3000.036.252333
GGTCGCG3000.033.8322567
TCCCCCC3250.033.4604728
TAGGCGG2206.202754E-1032.956674
GGGCGAC3100.032.7408947
CCCCCCG3350.032.4616559
CCTACAT1851.6401282E-731.3533673
GTCTATA950.00471708130.5370881
AGGTCGC3600.030.2102786
GTACAGG1504.384132E-529.0102351
GGCGACC3001.8189894E-1128.9990778
Produced by FastQC (version 0.11.2)