FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270850
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC4260.157282628761307No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4120.15211371607901053No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3790.13992985047074025No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT3370.12442311242385083No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3260.12036182388776076No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3150.11630053535167068No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3110.11482370315672881No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3020.11150083071810965No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT2980.1100239985231678No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC2800.10337825364592948No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC2760.10190142145098764No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG100.0070880824144.999634
GGGTAGG259.365648E-487.031911
TCCCCCC1350.059.0739258
TTCCGAT4550.054.1756862
CCGATCT4750.053.4209184
CCCCCCG1500.053.166539
CTTCCGA4650.053.0301971
CGATCTG2750.052.727145
TGGGGCG858.9006244E-751.176345
ACTCCCC2000.043.4998936
GGGCGAC1351.02118065E-842.9628527
TGCAGGG855.3312237E-542.646952
TATATAA700.001055289141.4284672
CTCCCCC2005.456968E-1239.87497
ATATACT1701.8189894E-938.3822564
TGTAGGA951.02692204E-438.1577952
CATACTC2350.037.0211833
CTATCCC1001.3887932E-436.249914
ACATATA1001.3887932E-436.249912
CGATCTA1001.3887932E-436.249915
Produced by FastQC (version 0.11.2)