FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169355
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACT30220.25843306780233544No Hit
ACCATACTCCCCCTGGAGCCCAAGCACTTTGATTTCTCGTAAGGTGCCGA19790.16923859734640037No Hit
GTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGAAGACTAACTAC18270.15623997844965815No Hit
ATGTAACATCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG17770.15196411697046663No Hit
GATTAATAGGGACAGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA16530.14135998050207166No Hit
CTCGTAAGGTGCCGAACGCGTCAAAAATGTAACATCGTCCGATCCCTAGT16160.13819584300746993No Hit
GAATAATAGAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGC15790.1350317055128682No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGACA15250.13041377511534136No Hit
GGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTT14970.1280192926869941No Hit
GTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCTGATTAATGAAAAC14530.12425653458530558No Hit
GTGCCGAACGCGTCAAAAATGTAACATCGTCCGATCCCTAGTCGGCATAG13230.11313929473940763No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG12640.10809377819396163No Hit
GAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGA12380.10587033022478204No Hit
ATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAAT11920.10193653766392584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA71250.067.98291
CCGATCT72750.065.971874
TTCCGAT74200.064.9757842
CGATCTG35750.063.474915
CGATCTA15600.054.374475
GATCTGG16200.047.8853386
CGATCTT11750.047.5101785
TCCGATC106500.045.677963
CGATCTC26150.043.8045465
GATCTAG8700.043.332916
GTGCTCT38700.040.096911
ATCTAGC6400.038.515257
TCTTCCG40100.037.7864155
TGCTCTT39850.037.6596072
ATCTGTG4250.037.5290457
GATCTGA12300.036.544366
TGTGCGC8150.034.6929138
GATCTGC23650.032.8009536
GATCTAC7350.032.5507056
ATCTGCG8350.032.125447
Produced by FastQC (version 0.11.2)