FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206649
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4330.2095340408131663No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3970.19211319677327254No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3340.16162671970345852No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3310.16017498270013406No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3300.15969107036569255No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2950.1427541386602403No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2510.12146199594481465No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT2490.12049417127593166No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG2470.11952634660704867No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2400.11613896026595823No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC2330.11275157392486776No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2330.11275157392486776No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2200.10646071357712837No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2150.1040411519049209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC805.851289E-754.3743678
GGTCGCG858.890802E-751.1758737
TCGCGCG752.5436771E-548.3327679
CCGATCT4050.042.9728624
TACACAG855.319494E-542.6568835
TTCCGAT4200.041.4381182
CTTCCGA4400.039.5641441
GTAAACT750.001478984638.6755754
CCTCCTA951.0246695E-438.1666832
CCTTTAA1209.496338E-636.267131
CCCTATT800.002028172836.2671281
ACACAGG800.002030585736.258356
ATATAGG850.002730640834.133771
GGGCGAC1301.6485927E-533.4611477
TAAGTTT1102.4262135E-432.9621355
AGGTCGC1153.1492265E-431.5290036
CTGACCT950.004713393730.5333484
AGTAAGG950.004713393730.5333482
TCTACTA1204.0415584E-430.2152942
GTTAGCG1204.047297E-430.20798145
Produced by FastQC (version 0.11.2)