FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107459
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3830.3564150047925255No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3280.305232693399343No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2990.2782456564829377No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2990.2782456564829377No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2850.26521743176467305No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2620.24381391972752398No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC2090.19449278329409356No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2070.19263160833434145No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2050.19077043337458938No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2020.18797867093496126No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG1990.1851869084953331No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT1960.18239514605570495No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1790.1665751588978122No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT1780.16564457141793615No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG1770.1647139839380601No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC1760.16378339645818407No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT1760.16378339645818407No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC1700.15819987157892776No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA1680.15633869661917568No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA1630.15168575921979546No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1610.14982458426004336No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA1590.14796340930029128No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT1580.14703282182041524No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC1580.14703282182041524No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA1570.14610223434053918No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG1360.1265598972631422No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1350.12562930978326617No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG1340.12469872230339012No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA1330.12376813482351408No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC1250.11632343498450572No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG1240.11539284750462968No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT1240.11539284750462968No Hit
ACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACTCC1230.11446226002475363No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG1210.11260108506500152No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC1190.11073991010524945No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA1170.10887873514549735No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT1160.10794814766562132No Hit
CTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAA1150.10701756018574526No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA1140.10608697270586923No Hit
CTCCTGGTCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAG1130.10515638522599316No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT1120.10422579774611712No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG1090.10143403530648899No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA1090.10143403530648899No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC203.85657E-4108.751
CGTCTTA451.225384E-680.55556145
CTTCCGA1550.079.516131
GTGTACA406.636179E-572.51
TTCCGAT1800.068.472222
CCGATCT1850.066.621634
CGATCTG1250.063.85
ACCATAC1200.060.4166681
ATACTCC1251.8189894E-1258.04
TCCCCCC1301.8189894E-1255.769238
CTCCCCC1450.055.07
ATATACA400.006036256454.3754
CTTAGAC400.006036256454.3753
CCCCCCG1353.6379788E-1253.7037059
CCATACT1405.456968E-1251.7857132
CATACTC1405.456968E-1251.7857133
ACTCCCC1600.049.843756
AGTATAC450.00961591748.3333325
TACTCCC1650.048.3333325
TCTTTAT450.00961591748.3333321
Produced by FastQC (version 0.11.2)