FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176940
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3450.1949813496100373No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3410.1927206962812253No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2830.15994122301345087No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2780.15711540635243584No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC2510.14185599638295468No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2430.13733468972533064No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2370.13394370973211256No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2320.13111789307109753No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG2280.12885723974228552No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2060.11642364643381936No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT1960.1107720131117893No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1890.10681586978636827No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT1860.10512037978975923No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1820.10285972646094721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG259.3646697E-487.021541
TATATAG400.006036770554.3884623
TCCCCCC1152.4647306E-950.4330148
CCCCTGG450.0096167148.3453031
CATACTA752.5382837E-548.3453032
GTATATG752.5382837E-548.3453031
AGGCCGT604.9348315E-448.331646
CTTCCGA3650.047.683041
CCGATCT3600.046.317824
CTCCCCC1307.2941475E-944.613827
CCCCCCG1307.2941475E-944.613829
ACTCCCC1307.2941475E-944.613826
TCTTCCG2500.043.4984785
ATACTAC855.3158383E-542.6576163
ATACTCC1557.3305273E-1042.0952954
TTCCGAT4000.041.697822
TTAAGGT1053.807163E-641.427124
ACTACCG700.001054464341.4271165
TAGGCAA700.001054464341.4271164
TCGCGCG907.458996E-540.2763679
Produced by FastQC (version 0.11.2)