FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79077
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1280.16186754682145252No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG990.12519443074471717No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA870.110019348230206No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC860.1087547580206634No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATAG259.354571E-487.02
AGACGCT300.001929130972.58
TGCACTG300.001929130972.55
CGATCTG1007.057679E-1058.05
TGTCAGG400.006030373354.3751
TTCTAAG400.006030373354.3752
CTCCCCC400.006030373354.3757
CATTATT400.006030373354.3752
TAATAAA450.0096065848.3333322
GTTTACA450.0096065848.3333321
ATATAAA450.0096065848.3333321
CCCCCCG450.0096065848.3333329
ATTTGGG450.0096065848.3333322
AATAAAC450.0096065848.3333323
CTTCCGA1900.045.7894741
CCGATCT1900.045.7894744
CTATGTT700.001050741541.428574
TTCCGAT2250.038.6666682
CCCTTAA1051.8383561E-434.523819
GTCTAAA850.002726809634.1176451
Produced by FastQC (version 0.11.2)