FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940525
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13000.13822067462321577No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11350.12067728130565375No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10230.1087690385688844No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC10130.10770580261024428No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT9800.10419712394673189No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA9470.10068844528321946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA20400.052.268941
CCGATCT21350.048.90314
CGATCTG11200.047.9055145
TTCCGAT22000.047.7903442
TCCGATC33500.031.6011523
TACTCCC8300.031.4482295
GTATAGC2558.731149E-1131.2902491
TCCCCCC7350.029.590998
CCCCCCG6950.029.2077969
CCGTGCC2003.2548087E-728.999179
CTCCCCC7600.028.6176017
GGGCGAC6150.028.2918747
ACCATAC9100.027.898651
ACACATA5800.027.500675145
TCTTCCG15900.027.3606175
CGATCTA5050.027.2793465
CATACTC9100.027.0914553
ACTCCCC8300.027.080426
CCATACT9000.026.5868112
TTATATA1658.410554E-526.3670882
Produced by FastQC (version 0.11.2)