FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399052
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8450.21175185188897688No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7240.18142998907410562No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6580.16489079117508496No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6500.1628860399145976No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6130.15361406533484356No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6030.15110812625923437No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5160.1293064563014344No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5130.12855467457875164No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5030.12604873550314244No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG4880.12228982688972867No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4540.11376963403265739No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4520.11326844621753557No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4260.10675300462095166No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT4230.10600122289826891No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT4210.10550003508314706No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT4110.10299409600753787No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4110.10299409600753787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT7200.061.4381034
CTTCCGA7750.057.1137731
CGATCTG4600.055.176065
TCGCGCG1900.053.4202659
TTCCGAT8350.052.10812
GGGGTGT657.322746E-444.625916
GTCGCGC2350.043.1908538
TATAGGT2400.042.3016433
AGGTCGC2400.042.3016436
CCCCTGC750.00148059938.675792
ATTATAC1502.5875124E-838.675793
GGTCGCG2700.037.592047
GTATACA800.002026548836.2812921
GCAGGGG800.002032795236.2585533
TCCCCCC3000.036.2494668
TATACAG1251.2611468E-534.808215
ACTCCCC3350.034.6350366
CCCCCCG2950.034.4062739
GGGCGAC2750.034.2722247
ATACTGG1301.6496335E-533.4694336
Produced by FastQC (version 0.11.2)