FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298858
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4560.15258082433797993No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4070.1361850778630654No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3790.12681607987739998No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3770.12614686573556672No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3610.12079315260090076No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3310.11075494047340208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG1450.054.999877
AGGTCGC1500.053.1665426
GTCGCGC1405.456968E-1251.78568
TCGCGCG1405.456968E-1251.78569
TATATAA1152.4738256E-950.4346662
ATAATAT604.939537E-448.333223
CTTCCGA4850.047.850951
GTATATA1850.047.042661
CCGATCT5150.045.048444
TTCCGAT5150.045.048442
CGATCTG3400.040.5146145
ATATAGG907.455211E-540.2911641
TATAGGT1951.6916601E-1037.17943
ATATAAC1803.1832315E-936.2499163
CTACCCC850.002738955234.117574
ATACCGC1102.4321173E-432.9544686
GTGTATG1352.1323562E-532.2329371
TAGGTCG2257.876224E-1032.222155
TATACCG1352.1372438E-532.222155
TTTTATA900.003624234632.2221452
Produced by FastQC (version 0.11.2)