FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84960
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1500.1765536723163842No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG1200.14124293785310735No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1110.1306497175141243No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC1110.1306497175141243No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1070.1259416195856874No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1000.11770244821092278No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC910.10710922787193974No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC910.10710922787193974No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT880.10357815442561205No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT860.10122410546139358No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT860.10122410546139358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG100.007068871145.073014
TAGTACC300.00192577572.5365144
CTAACGC451.1846443E-464.4769063
GCTAACG451.1846443E-464.4769062
GATCTAT350.00353991262.2107961
TCCCCCC350.003556482762.1375668
CCGATCT1200.060.4470944
GTGTAAG501.9889591E-458.0634041
CTATATA400.006005879554.434441
CAGGGTG400.006019904754.402385
CACTATG400.006019904754.402382
CAAGGGG400.006019904754.402383
ACTCCCC400.006019904754.402386
TCTATTC400.006019904754.402383
ATACACC400.006019904754.402384
CCCCCCG400.006033954754.3703659
GTACAAC553.184987E-452.7849121
TATATCC701.677754E-551.81184
TACACCA450.00958991948.3576775
CTTCCGA1402.6375346E-1046.6580961
Produced by FastQC (version 0.11.2)