FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444595
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGGCTAGCGGGCCATCGCGGCTTAGTAAACCATGGCAAACTCCGAA5920.13315489377973214No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG5740.12910626525264565No Hit
AGCGTATACGGTGGATGCCTTGGCGGTCAGAGGCGATGAAGGACGTGGCA5640.1268570271820421No Hit
GTATAGGGGAGGCGAAGGGAAACCGAGTCTGAATAGGGCGCATAGTCTCT5530.12438286530437814No Hit
CCCCTATACGGTTAAGCTTGCCACTGACAGTAAGTCGCTGACCCATTATA4930.11088743688075664No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4760.10706373216073056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT25850.061.701554
CTTCCGA26850.061.0236431
TTCCGAT26850.061.0236432
TCCGATC29300.055.1786653
CGATCTG14250.054.438095
CGATCTA5000.053.64955
CCTTACA701.6945414E-551.7852362
ATCTAGG858.914085E-751.1759957
CCTTAAT450.00963599548.3328862
CTAGGCC752.5484178E-548.3328829
ATCTATA657.3325064E-444.6149672
CGATCTT4750.044.2627455
ATCTGAC2500.040.5996257
GATCTGA4900.039.9486056
CGATCTC8750.039.7710575
CTAGACC750.001482566838.6663064
TCTAGAC750.001482566838.6663063
TACTATT750.001482566838.6663067
TAGCCCG1553.463174E-837.4190065
GATCTAG2550.036.960446
Produced by FastQC (version 0.11.2)