FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527434
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG66310.4341267773272037No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC51320.33598833075602613No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG49520.32420386085421693No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC40700.26645995833535197No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA31820.20832324015309334No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC29510.19319983711243824No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC29220.1913012280727023No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG29080.19038465819145048No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT28440.186194624448585No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC27890.18259381420080997No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG27880.1825283449235777No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC27670.18115349010169998No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT27050.17709439491329904No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC26990.1767015792499054No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG26340.17244607622980765No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA24020.15725720391192027No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGC22500.14730587377261473No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT22410.14671665027752426No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC22270.14580008039627243No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA21820.14285396292082014No Hit
GTCTGATAGCGCTTAAGCGCGAACTTCGAAAGGGGATCCGGTTAAAATTC21650.1417409852078715No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG21490.14069347677215513No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG21470.14056253821769057No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG21410.14016972255429694No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT21340.13971143761367105No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG21190.13872939845518695No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT20960.13722360507884465No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG20910.1368962586926833No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG20580.13473577254401828No Hit
ACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTT19900.1302838616922237No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA19800.12962916891990095No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC19420.12714133638507458No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT19370.12681398999891322No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG18610.12183832492926046No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA18380.12033253155291816No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA18270.11961236950336317No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA17880.11705906769130449No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT16970.11110136346316762No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT16850.11031573213638037No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT16670.10913728514619944No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG16670.10913728514619944No Hit
GGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGTCG16560.10841712309664445No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT16390.10730414538369579No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA16120.10553647489842442No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT15950.10442349718547576No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGG15930.10429255863101124No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG15810.10350692730422395No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC15590.10206660320511395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT106750.063.1668132
CTTCCGA108800.062.3969041
CCGATCT108700.061.5667234
CGATCTG53950.056.983395
GTATATA2050.056.6085741
TCCGATC126950.053.230083
CGATCTA21050.049.60035
CGATCTC39200.045.8711365
GATCTGA16600.044.9886556
ATCTGAC7200.039.270227
ATCTGAG8150.039.1404957
ATCTGGC8350.039.0712477
CTAACCG750.001483745238.6660659
CGATCTT21200.037.9630435
GATCTGG23350.037.5726976
GTGTTAA16900.035.6211131
ATCTGCG10050.035.3477067
ATCTGTG4000.034.4369667
GATCTAC12500.034.2228286
TATAAGG1301.6544147E-533.4643062
Produced by FastQC (version 0.11.2)