FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421923
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG48350.19963475304541062No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC47920.1978593043626903No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG40170.1658599385694756No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC36990.15272987621819523No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA33230.13720502262045492No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA31210.12886454276209441No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC31160.12865809524084787No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG30690.12671748854113032No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT30370.12539622440515244No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA28560.11792282413602745No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC28080.11594092793206061No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT27290.11267905709636516No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT25080.10355407665726779No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA24580.10148960144480232No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG24540.1013244434278051No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC24340.1004986533428189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT172750.057.3778652
CTTCCGA173900.057.057791
CGATCTG80900.056.911655
CCGATCT180650.054.946684
TCCGATC209100.047.5410043
CGATCTA34300.039.9524735
CGATCTC70800.037.994145
CGATCTT38750.033.8674775
ATCTGTG9250.032.91847
GATCTGG42800.032.8650256
GATCTAA10850.032.0765386
GATCTAG13900.031.29776
GATCTGA33000.030.3208316
TATACAG7250.029.0025355
CTAGGGG7650.028.4344463
GATCTCG36750.028.213356
ATCTAGC9350.027.9139967
GTGCTCT114900.027.586071
TGTTAAG24750.027.5388982
TCTTCCG113050.027.4504325