FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435970
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5590.12821983163979173No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4800.11009931876046516No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4690.10757620937220452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT7650.056.8664784
TTCCGAT8450.051.4826742
CTTCCGA8600.050.6079371
CGATCTG4850.041.8584185
TCCCCCC1604.5814886E-836.2482228
CGATCTA2103.765308E-1034.5260735
TCCGATC13400.033.546983
ATACTAC1801.2888086E-732.224343
CCCCCCG1801.2901182E-732.2206429
ACTACCG1153.1579795E-431.523815
CCAGCCC1153.160105E-431.5201958
GGGCGAC2151.8064384E-830.3473517
GTATATA1952.5896588E-729.7591971
ACACATA2303.472087E-828.374681145
GTCTAGA1306.457198E-427.8992461
TGTACCG1100.009662623526.3653685
CTCCCCC2207.472918E-726.3623437
CTACACC2859.855285E-925.440274
TATAAGC5500.025.04713
TAGACTG1500.001492353324.1682535
Produced by FastQC (version 0.11.2)