FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714508
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG19120.2675967238995225No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15610.2184720115100181No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC11970.16752786532830982No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG11560.16178965106058996No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA11230.1571710883573032No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10960.15339226432734132No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC10380.145274790485201No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC10100.14135601000968498No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA9660.13519792640530268No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8640.12092236895878002No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC8570.11994267383990104No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC8200.11476428535439771No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG8130.1137845902355187No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA8050.11266493867108555No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT7750.10846624530446126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT50350.064.93962
CTTCCGA50800.064.373361
CCGATCT52350.062.0431564
CGATCTG25700.058.112115
TCCGATC58050.056.4506263
TACAGGG2200.052.7266122
CGATCTA11600.049.9993745
CGATCTC18550.042.6005485
ACCTAAG750.001482174338.6715931
CGATCTT10600.036.93355
TGCTCTT28300.036.8899962
GATCTGG13850.036.642146
GTGCTCT28800.036.506391
GATCTGA8000.035.3433076
TCTTCCG30150.034.626435
ATCTTAC1707.8087396E-834.1219981
TATATGC1501.1334014E-633.832913
GATCTAC6450.033.7205056
GATCTCC7700.032.9541326
TTATACT1352.141535E-532.2218174
Produced by FastQC (version 0.11.2)