FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400413
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC9940.24824368839173552No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8820.22027256857294844No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC8380.20928391435842492No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8170.20403932939240232No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7200.1798143416922028No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6950.17357078816122354No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6850.17107336674883183No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6130.15309193257961154No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5770.1441012154950014No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT5640.1408545676588922No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5620.14035508337641386No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA5610.14010534123517468No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5550.13860688838773966No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT5470.1366089512578263No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT5180.12936642916189034No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC5170.12911668702065118No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT4880.12187416492471523No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA4860.12137468064223689No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4750.11862751708860601No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG4740.11837777494736684No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4730.11812803280612767No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG4660.11637983781745348No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA4490.11213422141638758No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC4260.10639015216788665No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC4210.1051414414616908No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG4160.10389273075549495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA8050.068.446021
CCGATCT7900.067.910224
TTCCGAT8200.067.193962
CGATCTG4050.057.2829675
TCTATAC902.0456355E-856.387923
TTATATA901.3217268E-648.3325042
GTAATAA752.5480798E-548.33251
CGATCTC3750.044.46595
CTATACA1151.409935E-744.1296734
GTATAAG700.001055971141.4278561
CGATCTA2500.037.6993525
TCCGATC14850.036.615533
GTATAAC800.002035331736.2493741
ATTACAG1051.8519245E-434.5232123
CCTGTCC1051.8519245E-434.5232128
ATACTGC1051.8519245E-434.5232126
GGGCGAC3950.033.0374037
TATATGG1551.4622983E-632.741372
CGAGGGG900.003625958732.221673
GTTATGC900.003625958732.221673
Produced by FastQC (version 0.11.2)