FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2b10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2b10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386560
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6820.17642798013245034No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5170.13374379139072848No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5150.13322640728476823No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5090.13167425496688742No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5020.1298634105960265No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4750.12287872516556292No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4580.11848096026490067No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4010.1037355132450331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA7250.063.016191
TAGGGGA350.003564874662.1427464
CCGATCT7750.057.064424
TTCCGAT8000.056.1873972
CGATCTG4500.048.333255
ACTGCGC1859.640644E-1139.1891178
CGATCTA2450.038.4693155
TCCGATC12350.036.983743
CTGCGCG2052.910383E-1035.365799
TATATCG1051.8516384E-434.5237465
GGACGTA850.002739938434.1175887
AGTGTGC850.002739938434.1175888
GTGCTCT5400.033.573441
GTCCTAA1956.9503585E-933.4701351
TAGTGTA900.003625531732.2221645
CTACACA1902.0710468E-730.526264
CATAGGG1204.053529E-430.2082792
GTGTAGG1453.4788056E-530.0077111
CATATAC1453.4849567E-529.9999493
TATGTGC1453.4849567E-529.9999496
Produced by FastQC (version 0.11.2)