FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403366
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6940.17205218089774546No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6290.15593778355141483No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5610.13907964478909973No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5520.13684842054114624No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5250.13015474779728584No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5150.12767560974400421No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4980.12346107505342542No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT4440.1100737295657046No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4370.10833833292840744No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT4360.10809041912307929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT6400.056.6451264
TTCCGAT6550.054.2476772
CTTCCGA7000.050.772921
TTAGGGG604.9365906E-448.3431743
CGATCTG3950.047.7253155
TAGGATG750.001481756638.6697434
GCATAGG800.00203067136.266371
GGACAGG800.002034383136.252886
TCCCCCC3050.035.6541528
CGCTACA1051.8468525E-434.5394021
TGCCGTA850.00273898734.120358145
CCCCCCG3200.033.9828649
GGGCGAC3250.033.460057
CATACTA1751.0048461E-733.14962
GGCGACC3350.032.4612438
GTGTTAA1801.2840428E-732.236771
CTCCCCC3600.032.2207877
TACTCCC4300.032.0374345
ACTCCCC3750.030.9357916
ACTACCG1652.3802932E-630.7600215
Produced by FastQC (version 0.11.2)