FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132234
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2940.22233313671219204No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2430.18376514360905666No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2130.16107808884250646No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2010.1520032669358864No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC1980.14973456145923136No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1850.13990350439372629No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC1830.13839103407595627No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1770.13385362312264623No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT1480.11192280351498103No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1430.108141627720556No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1410.10662915740278597No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT1380.10436045192613096No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1370.10360421676724595No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT1370.10360421676724595No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1350.10209174644947594No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT1350.10209174644947594No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC1340.10133551129059093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAG203.8585585E-4108.751
CAGGGGG406.641837E-572.54
CCTACAC451.190044E-464.444443
GTGCTAC451.190044E-464.444449
TCCCCCC1200.060.4166688
CTACTGC502.0038403E-458.04
CCCCCCG1301.8189894E-1255.769239
ACTCCCC1301.8189894E-1255.769236
CCAGGGG400.006039322454.3753
GTTAGAA400.006039322454.375145
TCCTACA400.006039322454.3752
AGGGGAG400.006039322454.3755
CTTCCGA2450.053.2653051
GTTCTAC450.00962078548.3333321
TCTTCCG2100.048.3333325
CTCCCCC1509.094947E-1248.3333327
GTTATTC450.00962078548.3333323
AGTGCTA657.313416E-444.6153838
TTCCGAT3000.043.52
ATACTCC1505.293259E-1043.54
Produced by FastQC (version 0.11.2)