FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629077
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC31100.4943750923972741No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT25200.400586891588788No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG24200.3846905863670107No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC20100.3195157349577238No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG19560.31093173013796405No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT19440.30902417351135075No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC18690.2971019445950178No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG17380.2762777847544895No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC16400.2606994056371477No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA16200.2575201445927923No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC15860.25211540081738804No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC15430.2452799895720238No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT15290.24305450684097493No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT15220.24194176547545054No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT14590.23192709318573085No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG14540.23113227792464197No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA14470.23001953655911755No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA14310.2274761277236332No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT14290.22715820161919764No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG14160.2250916819403666No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT14030.22302516226153554No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA13970.22207138394822892No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13950.22175345784379336No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG13910.22111760563492228No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT13160.20919537671858932No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT13090.20808263535306487No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG12590.20013448274217627No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT12390.1969552216978208No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT12290.19536559117564306No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC12230.19441181286233641No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA12080.1920273670790698No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA11970.19027877350467431No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA11920.18948395824358544No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT11400.18121787952826127No Hit
TTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGC11380.1808999534238257No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGA11370.18074099037160793No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11270.17915135984943018No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG10920.17358765302180815No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT10880.17295180081293704No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA10870.17279283776071927No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC10800.17168009639519488No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGC10380.1650036482020484No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC10110.1607116457921685No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT10100.16055268273995074No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACT9940.15800927390446637No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCG9840.15641964338228867No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT9400.14942526908470663No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9340.14847149077139998No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9300.14783563856252893No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA9240.14688186024922228No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTG9230.1467228971970045No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAA9040.14370259920486683No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG9040.14370259920486683No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT9030.14354363615264903No Hit
CCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGAC8810.14004644900385804No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC8700.13829785542946255No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8680.13797992932502698No Hit
ATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCG8670.1378209662728092No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA8640.1373440771161559No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCG8580.13639029880284925No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT8580.13639029880284925No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGC8560.1360723726984137No Hit
ATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAA8500.13511859438510707No Hit
GTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGA8490.1349596313328893No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8440.13416481607180045No Hit
CCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGG8320.13225725944518715No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCG8170.12987281366192055No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAA8110.12891903534861393No Hit
TCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGC8070.12828318313974282No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG8000.1271704417742184No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC7900.12558081125204068No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG7850.12478599599095182No Hit
CGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCA7780.12367325462542741No Hit
ACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGT7760.12335532852099187No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT7740.12303740241655632No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAG7720.12271947631212077No Hit
ACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTT7690.12224258715546746No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAG7670.12192466105103192No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC7650.12160673494659636No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7560.12017606747663642No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA7560.12017606747663642No Hit
GGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTACCCGAT7510.11938125221554753No Hit
GGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAG7480.11890436305889422No Hit
CTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGC7470.11874540000667644No Hit
CTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGAT7450.1184274739022409No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC7390.11747369558893428No Hit
GAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGA7230.11493028675344989No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC7190.1142944345445788No Hit
TCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAAT7120.1131816931790544No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG7120.1131816931790544No Hit
CCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGT7040.1119099887613122No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAA7000.1112741365524411No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6900.10968450603026339No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGG6880.10936657992582785No Hit
GATTACGGTGGCGGCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGT6880.10936657992582785No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAA6860.1090486538213923No Hit
ACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGG6830.10857176466473896No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG6800.10809487550808565No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC6790.1079359124558679No Hit
CTTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTAACG6780.10777694940365011No Hit
ACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCC6710.10666420803812571No Hit
ACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCCAC6700.10650524498590791No Hit
CCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCG6690.10634628193369015No Hit
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC6630.10539250362038351No Hit
CTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACG6610.10507457751594797No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGT6590.10475665141151241No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGG6570.10443872530707687No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAA6560.1042797622548591No Hit
TGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCAC6530.10380287309820577No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT6510.10348494699377025No Hit
TCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG6500.10332598394155246No Hit
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCC6450.10253116868046361No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA6440.10237220562824582No Hit
TAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAG6410.10189531647159251No Hit
GTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCT6390.10157739036715696No Hit
ACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGT6330.10062361205385031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGG1400.062.150486145
ATGTTTC1650.061.5129287
TAGATGT1900.057.2347764
CGATCTA4100.056.583325
TTCCGAT24500.056.5183682
ATCTAGC902.0485459E-856.386857
CTTCCGA24900.055.6369741
ATCTGTG1450.054.998017
CATTAGG400.00603930354.398981
ATATAGG400.006050711554.3730353
GATCTAG1600.054.3730356
CCGATCT26150.053.2294354
TCTACTC553.2186575E-452.7253658
ATCTTAC701.695623E-551.783847
CTTAGAT2200.049.430032
GATATGG450.00962071648.3546451
CTCTAGG450.00962071648.3546451
CGATTAT450.00963281248.339268145
ACAGTGT450.00963886548.3315858
TACAGGA604.944556E-448.3315852
Produced by FastQC (version 0.11.2)