FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574057
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC7920.1379653936804185No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG7790.13570081019829042No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG6790.11828093725884364No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG6760.11775834107066023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT33350.067.8300864
TTCCGAT33800.066.927022
CTTCCGA35000.064.649281
TCCGATC36250.062.403673
CGATCTG18850.056.1571545
CGATCTC12300.048.3361785
CGATCTT6700.046.532595
CGATCTA6900.043.082255
GTATATA855.3285592E-542.6607171
TATATAC1252.7149872E-740.602393
GTTTATA750.001480527238.679051
GTTATAT951.02640624E-438.1701161
GATCTCA4850.037.3733376
GATCTCC5600.034.9574176
ATATACC1251.2636412E-534.8020484
ATCTGGC4450.034.2125177
GATCTGG9600.033.9863786
TAATGTG1102.4352667E-432.9536139
ATATAGG1352.1397598E-532.224122
GATCTTT2950.031.9510366
Produced by FastQC (version 0.11.2)