Basic Statistics
Measure | Value |
---|---|
Filename | H2KH2AFXY_n01_p2a12.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 722040 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG | 1530 | 0.21189961774970914 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT | 1520 | 0.21051465292781565 | No Hit |
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC | 1123 | 0.15553154949864273 | No Hit |
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG | 1102 | 0.15262312337266634 | No Hit |
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC | 922 | 0.12769375657858292 | No Hit |
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA | 849 | 0.11758351337876018 | No Hit |
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG | 768 | 0.10636529832142264 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTCCGA | 5315 | 0.0 | 53.21616 | 1 |
TTCCGAT | 5415 | 0.0 | 52.22255 | 2 |
CCGATCT | 5650 | 0.0 | 50.043533 | 4 |
CGATCTG | 2720 | 0.0 | 46.64455 | 5 |
CTAAGAC | 95 | 1.9194104E-6 | 45.791992 | 3 |
TCCGATC | 6550 | 0.0 | 43.17031 | 3 |
CGATCTA | 1170 | 0.0 | 37.178955 | 5 |
CGATCTC | 2185 | 0.0 | 34.839317 | 5 |
GATCTAA | 320 | 0.0 | 33.983887 | 6 |
GTGTTAA | 995 | 0.0 | 33.528717 | 1 |
CCTATAG | 90 | 0.0036216755 | 32.23292 | 1 |
ATACCTA | 115 | 3.161501E-4 | 31.521286 | 5 |
CGATCTT | 1180 | 0.0 | 30.719896 | 5 |
GATCTGG | 1450 | 0.0 | 30.499563 | 6 |
ATCTGAG | 345 | 0.0 | 29.41987 | 7 |
TGTTAAG | 1090 | 0.0 | 29.26969 | 2 |
TCTAACG | 235 | 4.288995E-8 | 27.769407 | 2 |
GTTAAGA | 1155 | 0.0 | 27.620565 | 3 |
TCCTACA | 290 | 3.947207E-10 | 27.503416 | 2 |
TGCTCTT | 3485 | 0.0 | 26.839775 | 2 |