FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2a12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2a12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722040
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG15300.21189961774970914No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT15200.21051465292781565No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11230.15553154949864273No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG11020.15262312337266634No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC9220.12769375657858292No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8490.11758351337876018No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG7680.10636529832142264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA53150.053.216161
TTCCGAT54150.052.222552
CCGATCT56500.050.0435334
CGATCTG27200.046.644555
CTAAGAC951.9194104E-645.7919923
TCCGATC65500.043.170313
CGATCTA11700.037.1789555
CGATCTC21850.034.8393175
GATCTAA3200.033.9838876
GTGTTAA9950.033.5287171
CCTATAG900.003621675532.232921
ATACCTA1153.161501E-431.5212865
CGATCTT11800.030.7198965
GATCTGG14500.030.4995636
ATCTGAG3450.029.419877
TGTTAAG10900.029.269692
TCTAACG2354.288995E-827.7694072
GTTAAGA11550.027.6205653
TCCTACA2903.947207E-1027.5034162
TGCTCTT34850.026.8397752