FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2a11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2a11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336811
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8280.24583520134437414No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7020.2084254967919694No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6230.18497020584244578No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5970.17725074299829877No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5770.1713126946566472No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5370.1594365979733441No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5280.1567644762196009No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5250.15587376896835317No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4880.14488837953629782No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT4850.14399767228505006No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT4610.1368720142750682No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC4230.12558972242593028No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC4020.11935477166719614No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3890.11549504024512262No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3820.11341672332554459No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3770.11193221124013171No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG3750.11133840640596655No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3680.1092600894863885No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT3650.10836938223514078No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT3570.10599416289848015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA6300.064.482411
CCGATCT6400.061.1715624
TTCCGAT6700.060.60572
CGATCTG3650.057.6024445
GCCTATT450.00961123948.3618051
GGGCGAC3150.048.3330887
CCTGGGC657.3298113E-444.6151588
CCTATAG657.3298113E-444.6151583
GGGTACC855.334083E-542.646846
CAGGGGC855.334083E-542.646844
TCCCCCC3300.041.742218
TCTTCCG3800.040.0655865
CATACTC4450.039.1009253
CTAGACT1303.6887832E-739.038264
TCCTAGA750.001480944538.6722072
CTCCCCC3750.038.666477
TAGGGGT750.001482025738.666474
CCCCCCG3600.038.2636959
TCTAGAC951.0274702E-438.15773
ATACTCC3800.038.15774
Produced by FastQC (version 0.11.2)