FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p2a10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2a10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521484
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7820.14995666214112036No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7010.1344240667019506No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6540.12541132613848172No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6170.11831618995021898No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5840.11198809551203873No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5650.10834464719914705No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC5510.10566000107385845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT9900.058.595932
CTTCCGA10200.056.1777841
CCGATCT12300.046.568584
GTATAAT1453.8198777E-1045.020691
GGGCGAC3600.044.304687
CGATCTG6750.044.0403865
TCCGATC17550.033.0509453
GCGACCC4800.031.7181199
TCGCGCG3000.031.4160449
GGCGACC4900.031.0708148
TGGCACG950.004723484630.5286395
CAGGGGC1504.392441E-529.0022074
GGGGCGA5300.028.7286016
GTCTAGG1306.4613624E-427.8974381
GGTCGCG3403.6379788E-1227.720047
GAATAGA1606.800651E-527.21
ATACTAG1606.800651E-527.21
GTTATAC1606.800651E-527.21
TACGAGT3750.027.0687264
TAATATA1358.0693874E-426.8564682
Produced by FastQC (version 0.11.2)