FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26189797
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG93850.060.44395
CGGATAG60450.059.8637855
CGTATAA101050.058.83345
CTATACG55550.058.1017846
GGTATAG187800.057.8449555
TTATAGT254700.057.6684956
ATTATAG222650.057.576345
TATAGGG226900.057.4977
CTATAGG105800.056.8524326
GGATAGT132850.056.7928626
GTATAGG209450.056.318656
TATAGGC135950.056.1128777
TATAGCG111250.055.3844267
TATAGTC133400.055.038427
GGGATAG150200.054.6045535
TTATAGC147150.054.1934976
GTATAGC113800.054.0221676
TTATAAG194600.053.8672376
TTATACG114750.053.8184436
TATACGC59700.053.6517757
Produced by FastQC (version 0.11.5)