FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083503
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA7050.063.0422865
GTTATAG18600.063.030285
CGTATAG7650.061.75755
TATAACG6300.061.6594737
CTATACG3850.060.901986
ATTATAG17800.060.751345
CGATACG1850.060.5334746
ATAACGC1850.060.5306858
GTTATAA14350.060.4807435
GTATAAG11450.060.2113156
GGTTATA18850.060.1521344
TTATAGC10900.060.0388646
CTATAGG9600.059.784696
CGTTATA10750.059.574444
CCGATAG2300.059.3409045
ATAGGCG3750.058.7904248
TTATAGG23750.058.3510866
TTATAGA13900.058.158686
GGTATAG15800.058.0312045
CGATAAG3800.058.0195436
Produced by FastQC (version 0.11.5)