FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073125
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG3150.066.6630866
CGTATAA13150.062.278025
CGGATAG13000.061.9197465
ATTATAG39100.060.95585
GTTATAG38250.060.93795
CGTTATA18500.060.9156464
CGATAAG8250.060.6634066
TATAGCG18450.060.321967
CTATACG9650.060.204026
GCGGATA13100.060.1112754
GGTATAG34850.059.9537355
CGGATAA10050.059.897285
TATAACG11000.059.49687
GTTATAA25950.059.476575
CGTATAG19200.059.0593265
CTATAGG21400.058.7117966
TTATAGC23900.058.5742536
GGATAGT26100.058.3301966
GTATAAG25200.058.191326
TATAGGG49900.058.1431667
Produced by FastQC (version 0.11.5)