FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155489
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAA10950.063.289395
CGTTATA20950.062.817944
GTTATAG37050.062.7277455
CGATACG3450.061.8857356
CTATACG9450.061.1127746
CGTATAA16600.060.724545
TATAACG12850.060.4643637
ATTATAG39250.060.192725
TATAGCG18300.060.0520977
GTATAAG24600.059.96
GGTATAG33200.059.8811455
CGGATAG14000.059.7516255
GGTTATA38350.059.2323764
TATACGC9100.059.2282567
TTATAGC23700.059.221026
TTATAAC16000.059.0641066
CGTATAG18400.058.588555
GTTATAA28400.058.5403255
TTATATG50800.057.944496
CGATAAG8350.057.8458826
Produced by FastQC (version 0.11.5)