FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173723
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG39050.063.278975
CGTATAA16400.062.104765
CGATACG3900.061.9241756
CGTATAG18000.061.834435
ATTATAG39950.061.7658045
TTATAGC23700.061.583376
TTATAAC14800.061.4875766
CGGATAG13450.061.1535035
CTATAGG19100.061.0220266
GTATAAG25450.060.9244586
CGTTATA19550.060.6916164
GTTATAA27700.060.6508945
GCGGATA14150.060.6017844
TTATAGT46550.060.1514436
CGGATAA9700.059.897975
TTATACG19950.059.8256236
GGTATAG35750.059.525535
CTTATAA18950.059.1039545
TTTATAG49950.059.0701185
GGTTATA42100.059.027184
Produced by FastQC (version 0.11.5)