FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3451137
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG52850.062.9728435
CGTATAA24100.062.876545
CTATACG13000.061.6467136
GGTATAG43650.061.4132845
CGGATAG15000.061.1262635
CGTATAG22250.060.712085
TATAGCG26550.060.236987
CGATAAG12300.060.03376
CGTTATA28350.059.8696174
TTATAGC36900.059.844026
TATAACG17400.059.532487
TTTATAG66150.059.4111145
ATTATAG59400.058.9746635
ATAGGCG12500.058.7923438
CTATAGG28450.058.4290926
GGGTATA45900.058.4028244
CTTATAC18450.058.231745
GCGGATA17950.057.9041634
TTATACG27300.057.813826
GGTTATA52000.057.6760184
Produced by FastQC (version 0.11.5)