FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135659
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG6850.061.820625
GGATAGT15800.061.7995956
CGGATAA4550.061.5342835
CGATAAG4100.061.4592366
ATTATAG21900.061.2058875
TATACGC5100.061.074287
GTTATAG22000.060.6095245
TTATACG10650.060.4653746
TTATAGT26250.060.3958976
TATAACG4300.060.228477
GTATAAG13300.059.9959266
CGTATAA7350.059.9959225
TTTATAG28400.059.89035
GGTATAG18850.059.5980725
CGATAGT6300.059.4404076
CCTATAC3300.059.3899085
ATAGGGC6750.059.1070988
CCTATAG5050.058.906895
CGCTATA5550.058.6446654
GCGGATA7800.058.3293724
Produced by FastQC (version 0.11.5)