FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3484016
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA24000.063.8757865
CGGATAG21050.063.0174035
GTTATAG63650.062.6323475
CGTATAG30100.062.0937845
CGTTATA32850.061.583714
TTATAAC23550.061.3807956
GGTATAG57250.061.380675
CGGATAA15350.061.108245
GTATAAG42850.061.0159156
ATTATAG62600.060.9991535
CGATACG5250.060.6674166
GGATAGT44600.060.26986
TTATAGT77500.059.929776
TATAACG19000.059.6840867
TTTATAG76300.059.633765
TATAGCG31850.059.4504287
TTATAGC36300.059.3946726
TTATATG84100.059.3051346
GGTTATA64400.059.131164
GCGGATA22850.059.1254544