FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5315674
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG69000.062.741395
GTTATAG80700.061.364395
CGTATAA36650.060.349915
CGGATAG26750.060.312975
CGTATAG36100.060.2999235
CGTTATA45950.059.940834
GGATAGT53050.058.77956
CGATAAG17750.058.7558986
TATAACG25250.058.62687
ATAGGCG17750.058.162218
TATAGCG41050.058.056547
CCGATAG13150.058.0182045
GTATAAG59900.058.017096
GGTTATA81200.057.797144
GGGTATA70250.057.639554
CTATAGG45850.057.6290136
GTATAGG85600.057.6064076
TTTATAG102450.057.5259365
ATTATAG91650.057.4695175
TTATAGT105000.057.4287726
Produced by FastQC (version 0.11.5)