FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546469
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG17950.061.612995
GTTATAG63700.061.1512535
CGTTATA31400.060.077084
CGATAAG11350.059.821266
CGTATAA22250.059.4581575
TTATAGG87950.059.0537876
GGTATAG54400.058.545325
GGATAGT46650.058.3683786
TATACGC13650.058.2010237
TATAACG15300.058.1004837
ATTATAG70750.057.5311365
CGGATAA15850.057.4108165
GCGGATA21600.057.3586854
GTTATAA45800.057.3119855
TTTATAG92650.057.2290955
CTATACG14500.056.9631046
TTATAGT84200.056.9453356
TATAGCG29100.056.765767
TTATAAC22700.056.737696
TTATAGC44000.056.6339726
Produced by FastQC (version 0.11.5)