FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3797324
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG21050.064.004847
GTTATAG68900.063.031375
CGGATAG21000.062.9907345
CGTATAA24400.062.1015175
CGTATAG30500.062.0728385
ATTATAG71450.061.810365
CGTTATA35000.061.790914
TTATAGC46500.061.5608336
CTATACG15850.061.1581956
TTATAGT81500.060.9726226
GTTATAA47400.060.908765
GATAGTC15550.060.762767
TATAGCG33200.060.713967
TTTATAG95200.060.3220065
GCGGATA23800.059.8441354
TTATAAC24600.059.7473076
GGATAGT46500.059.679396
CGATAAG15200.059.6293836
CGGATAA16850.059.6054765
CGATACG6050.059.5780146
Produced by FastQC (version 0.11.5)