FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904884
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG17300.062.511115
GTTATAG61250.061.596715
CGATACG5400.060.9226726
GGTATAG51450.060.7450565
CGTATAG27950.060.604995
CGGATAA17300.060.0834925
CCGATAG9750.059.586565
CTATACG16300.059.0459256
ATTATAG69200.058.7685365
TATAACG18950.058.7281157
CGTATAA27500.058.4150625
GGATAGT39850.058.40346
GTATAAG43000.058.1131746
CGATAAG13250.057.8459666
CTATAGG31100.057.842576
CGTTATA34500.057.721554
GGTTATA62200.057.6175044
TATACGC15250.057.601277
TTATAGC41100.057.3935286
TTTATAG80150.057.245855
Produced by FastQC (version 0.11.5)