FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5075925
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG91250.061.2506945
TATAACG21850.061.0256587
CTATACG17850.060.9763076
CGTATAA32100.060.2919125
GGATAGT62600.060.155726
TATACGC15950.059.9019477
CGGATAG26700.059.64025
CGTTATA44900.059.4726334
GGTATAG77700.059.140185
ATTATAG94700.058.8714525
CGATAAG18550.058.6753166
GCGGATA29550.058.625514
CGATACG7600.058.482926
GGTTATA95150.058.151654
GTATAAG55750.058.0678566
GTTATAA67100.057.7383085
TTATAGC55950.057.672636
TTTATAG122950.057.4707345
TATAGCG40900.057.4166877
TTATAGT114150.057.3023386
Produced by FastQC (version 0.11.5)