FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4586399
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG75800.062.2837835
CGTATAA34250.062.2284555
GGTATAG61700.062.1666765
CGGATAG24450.061.6922575
CGTATAG33700.061.3747635
CGTTATA40550.060.845794
TATAACG25550.059.9937487
GTTATAA60950.059.9458435
CGGATAA21850.059.903535
TATAGCG33800.059.74237
GGATAGT45950.059.7121666
CGATAAG16900.059.640066
ATTATAG82250.059.399365
CTATACG20450.059.383866
TTATAAC31400.059.2944766
GCGGATA28450.058.6770634
TTATACG37500.058.60856
TTTATAG92000.058.506055
CTATAGG37300.058.2659766
TTATAGT88700.058.1970066
Produced by FastQC (version 0.11.5)